Purification and Some Properties ofSporobolomycesRed Yeast Cell Wall Lytic Enzyme

Extracellular cell wall lytic enzyme from Staphylococcus aureus: purification and partial characterization.

An autolysin obtained from culture fluid of Staphylococcus aureus strain 8507 was purified 3,000-fold. One milligram of this preparation (S-5DL) will solubilize 12 mg of cell wall in 1 hr. The major activity is N-acetylmuramyl-l-alanine amidase. Recovery of lytic activity in the purified preparation was repeatably only 20% of the starting level. This suggests that other cell wall lytic enzymes ...

Yeast lipases: enzyme purification, biochemical properties and gene cloning

Lipases are placed only after proteases and carbohydrases in world enzyme market and share about 5% of enzyme market. They occur in plants, animals and microorganisms and are accordingly classified as plant, animal and microbial lipases. Wherever they exist, they function to catalyze hydrolysis of triglycerides to glycerol and fatty acid. Like carbohydrases and proteases, lipases of microbial o...

Yeast pyruvate kinase. I. Purification and some chemical properties.

Purification Akd Some Properties of the Enzyme from Rabbit Hikdbrain

Tryptophan hydroxylase from rabbit hindbrain has been extensively purified. It is estimated that the enzyme is between 85 and 90 % pure and has a molecular weight of 230,000. Sodium dodecyl sulfate gel electrophoresis shows that the enzyme is composed of two subunits very close in molecular weight (57,500 and 60,900). The substrate specificity and the reaction stoichiometry catalyzed by the enz...

Purification and properties of a germination-specific cortex-lytic enzyme from spores of Bacillus megaterium KM.

Two peptidoglycan-lytic enzyme activities were isolated from spores of Bacillus megaterium KM. Surface-bound lytic enzyme was extracted from dormant spores and hydrolysed a variety of peptidoglycan substrates including isolated spore cortex, but did not cause refractility changes in permeabilized spores. Germination-specific lytic enzyme activity appeared early in germination and had minimal ac...